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Downstream

Downstream process development
Prestige Biologics develops the optimal purification process by utilizing the patented technology
“Non-Protein A purification process of antibody”.
Secure economical
and efficient
downstream process
  • Design of Experiment (DoE) studies
  • Resin screening
  • UF/DF development
  • Determination of In-Process Control & In-Process Testing (IPC & IPT)
Protein A purification process vs Non-Protein A purification process
Protein A purification process
  • 01 Protein A affinity
    Chromatography
  • 02 Viral Inactivation
  • 03 Intermediate
    Chromatography
  • 04 Polishing
    Chromatography
  • 05 Virus Filtration
  • 06 Ultrafiltration
    / Diafiltration
Product recovery : ≥ 70%
Purity : ≥ 95%
Non-Protein A purification process
  • 01 Ion Exchange
    Chromatography
  • 02 Viral Inactivation
  • 03 Intermediate
    Chromatography
  • 04 Polishing
    Chromatography
  • 05 Ultrafiltration
    / Diafiltration
  • 06 Virus Filtration
Product recovery: ≥ 71%
Purity: ≥ 99%

Detailed purification steps may differ depending on the product.
Chon and Zarbis-Papastoitsis, 2011; Pinto et al., 2015; Xenopulos, 2015)

Prediction Profiler and Contour Profiler for Buffer of Non-protein A Chromatography